Detection of Dengue Viral Rna in Mosquitoes (aedes Sp.) by Nucleic Acid Sequence-based Amplification (nasba) and Reverse Transcriptase-polymerase Chain Reaction (rt-pcr)
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چکیده
of the Joint International Tropical Medicine Meeting (JITMM). Bangkok, Thailand. 29 November-1 December 2004:106. EVALUATION OF A SINGLE-DILUTION PLAQUE REDUCTION NEUTRALIZATION TEST (PRNT) AS A DIAGNOSTIC TOOL FOR DETECTING INTERCURRENT DENGUE VIRUS (DENV) INFECTIONS Thomas SJ, Nisalak A, Halstead S, Endy T, Lutthiwongsakorn N, Poolpanichupatam Y, Sawatwong P, Changnak S and Mammen MP Jr Studies of DENV transmission require diagnostic tools capable of detecting symptomatic and asymptomatic infections. The hemagglutination-inhibition (HAI) test is valuable for the diagnosis of DENV infections, but possesses low specificity. Since HAI antibody may wane, the HAI test as a DENV surveillance tool may require frequent cohort serum sampling. The PRNT is considered the gold standard bioassay for measuring DENV specific neutralizing antibody. The PRNT is labor-intensive and requires affinity maturation to provide specificity. Previously reported studies have relied on a single-dilution neutralization test (SDNT) to detect intercurrent
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